Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados BABT (1) BJM (1) Autor Syed,Quratulain (2) Adnan,Ahmad (1) Ahmad,Waqar (1) Irfan,Muhammad (1) Mushtaq,Zareena (1) Nadeem,Muhammad (1) Naz,Mammona (1) Shabbiri,Khadija (1) Mais... Palavra-chave Characterization (1) Complex II (1) Cytochrome b (1) Detergent stability (1) Kinetics (1) Protease (1) Proteus mirabilis (1) Purification (1) Rhizopus oryzae (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2015 (1) 2010 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Isolation and purification of complex II from proteus mirabilis strain ATCC 29245 BJM Shabbiri,Khadija; Ahmad,Waqar; Syed,Quratulain; Adnan,Ahmad. A respiratory complex was isolated from plasma membrane of pathogenic Proteus mirabilis strain ATCC 29245. It was identified as complex II consisting of succinate:quinone oxidoreductase (EC 1.3.5.1) containing single heme b. The complex II was purified by ion-exchange chromatography and gel filtration. The molecular weight of purified complex was 116.5 kDa and it was composed of three subunits with molecular weights of 19 kDa, 29 kDa and 68.5 kDa. The complex II contained 9.5 nmoles of cytochrome b per mg protein. Heme staining indicated that the 19 kDa subunit was cytochrome b. Its reduced form showed absorptions peaks at 557.0, 524.8 and 424.4 nm. The α-band was shifted from 557.0 nm to 556.8 nm in pyridine ferrohemochrome spectrum. The succinate:... Tipo: Info:eu-repo/semantics/article Palavras-chave: Proteus mirabilis; Complex II; Cytochrome b. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300032 Kinetics Study of Extracellular Detergent Stable Alkaline Protease from Rhizopus oryzae BABT Mushtaq,Zareena; Irfan,Muhammad; Nadeem,Muhammad; Naz,Mammona; Syed,Quratulain. In this study, extracellular alkaline protease was produced from Rhizopus oryzae in submerged fermentation using dairy waste (whey) as a substrate. Fermentation kinetics was studied and various parameters were optimized. The strain produced maximum protease at initial medium pH of 6.0 medium depth of 26 mm, inoculum size of 2% at incubation temperature of 35ºC for 168 h of fermentation. Alkaline protease was purified to homogeneity by ammonium sulphate fractionation followed by sephadex G-100 chromatography. The molecular mass of alkaline protease was 69 kDa determined by 10% SDS-PAGE. The optimum pH and temperature of alkaline protease was 9.0 and 40ºC, respectively. Metal profile of the enzyme showed that the enzyme was non-metallic in nature. The Km ,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Kinetics; Purification; Characterization; Protease; Detergent stability; Rhizopus oryzae. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200175 Registros recuperados: 2 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco